Archaeological Museum, Plaza de America
Opening hours: Tuesdays 15-20, Wed-Sat 9-20, Sundays 9-14.
On display:  interesting collection of Visigoth, Roman, Moorish and Christian artefacts. Of special importance are the "Carambol-Treasure" dating back from the 650 b.c. and relating to the old Iberian tribe the Tartessos.

Museum of Fine Arts, Plaza del Museo, 9.
Opening hours: Tuesdays 15-20, Wed-Sat 9-20, Sundays 9-14.
Located in a former convent, a building from 1839, it  hosts one of the most important collections of Spanish paintings from the 15th to the 20th century including works by Murillo, Ribera, El Greco, Zurbarán and Valdés Leal.

Museum of Contemporary Arts, Santo Tomás, 5.
Opening hours: Tues-Fri 10-20, Sat-Sun 10-14.
On display: paintings and sculptures of Spanish artist from the 20th century such as Barbadillo, Caballero, Cortijo, Gordillo, Gerrero and Mateos.

Cathedral Museum, Plaza de la Virgen de los Reyes, s/n.
Opening hours: Mon-Sat 11-17, Sundays 14.30-18.
Located in the chapel and the sacristy halls of the Cathedral, it features religious paintings, sculptures and manuscripts by the likes of  Goya, Murillo, Pedro de Campaña and Luis de Vargas.

Museum of Popular Art and Customs, Plaza de America, s/n.
Opening hours: Tuesdays 15-20, Wed-Sat 9-20, Sundays 9-14.
On display: a collection of ethnographic themes from Andalucia from the 17th to the 20th centuries.

Bullfight-Museum, Paseo de Cristóbal Colon, 2. 
Opening hours: Mon-Sun 9.30-14 and 15-19.
Located in the bullring La Real Maestranza,  the Museum features information about the history and origins of bullfighting and a part dedicated to bullfighting legendary figures.

Museum of Navigation "The Golden Tower", Paseo de Cristóbal Colon
Opening hours: Tues-Fri 10-14, Sat-Sun 11-14.
Located in an Almohade tower built in 1222, the museum displays maritime objects.

Cartuja Museum, Virgen de la Consolación, 4.
Opening hours: Mon-Fri 10-14 and 17-21.
On display: an interesting collection of old horse carriages.